English
norskBlar i forfatter "Larsen, Anett Kristin"
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Autofluorescence in freshly isolated adult human liver sinusoidal cells
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-12-13)Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment ... -
Brucella pinnipedialis in hooded seal (Cystophora cristata) primary epithelial cells
(Journal article; Tidsskriftartikkel; Peer reviewed, 2016-01-25)Background: Marine Brucella spp. have been isolated from numerous pinniped and cetacean species, but pathological findings in association with infection with Brucella pinnipedialis in pinnipeds have been sparse. The capacity of brucellae to survive and replicate within host macrophages underlies their important ability to produce chronic infections, but previous work has shown that B. pinnipedialis ... -
Concomitant Temperature Stress and Immune Activation may Increase Mortality Despite Efficient Clearance of an Intracellular Bacterial Infection in Atlantic Cod
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-11-04)The environmental temperature has profound effects on biological systems of marine aquatic organisms and plays a critical role in species distribution and abundance. Particularly during the warmer seasons, variations in habitat temperature may introduce episodes of stressful temperatures which the organisms must adapt to and compensate for to maintain physiological homeostasis. The marine environment ... -
Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua)
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-07-05)Inclusion of new environmental toxicants increase with the amount of plant ingredients substituting marine proteins and oils in feed for farmed Atlantic salmon (Salma salar). Agricultural pesticides like chlorpyrifos-methyl, present in commercial salmon feeds, may affect salmon immune and detoxification responses. Atlantic cod (Gadus morhua), surrounding the net pens, grazing on feces and uneaten ... -
Differences in PAR-2 activating potential by king crab (Paralithodes camtschaticus), salmon (Salmo salar), and bovine (Bos taurus) trypsin.
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013-07-20)Background: Salmon trypsin is shown to increase secretion of the pro-inflammatory cytokine interleukin (IL)-8 from human airway epithelial cells through activation of PAR-2. Secretion of IL-8 induced by king crab trypsin is observed in a different concentration range compared to salmon trypsin, and seems to be only partially related to PAR-2 activation. This report aim to identify differences in ... -
Effects of Agricultural Pesticides in Aquafeeds on Wild Fish Feeding on Leftover Pellets Near Fish Farms
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-09-26)Screening has revealed that modern-day feeds used in Atlantic salmon aquaculture might contain trace amounts of agricultural pesticides. To reach slaughter size, salmon are produced in open net pens in the sea. Uneaten feed pellets and undigested feces deposited beneath the net pens represent a source of contamination for marine organisms. To examine the impacts of long-term and continuous dietary ... -
Entrance and survival of Brucella pinnipedialis hooded seal strain in human macrophages and epithelial cells
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)Marine mammal Brucella spp. have been isolated from pinnipeds (B. pinnipedialis) and cetaceans (B. ceti) from around the world. Although the zoonotic potential of marine mammal brucellae is largely unknown, reports of human disease exist. There are few studies of the mechanisms of bacterial intracellular invasion and multiplication involving the marine mammal Brucella spp. We examined the infective ... -
Experimental challenge of atlantic cod (Gadus morhua) with a brucella pinnipedialis strain from hooded seal (Cystophora cristata)
(Peer reviewed; Journal article; Tidsskriftsartikkel, 2016-07-14)Pathology has not been observed in true seals infected with Brucella pinnipedialis. A lack of intracellular survival and multiplication of B. pinnipedialis in hooded seal (Cystophora cristata) macrophages in vitro indicates a lack of chronic infection in hooded seals. Both epidemiology and bacteriological patterns in the hooded seal point to a transient infection of environmental origin, possibly ... -
Infection of liver sinusoidal endothelial cells with Muromegalovirus muridbeta1 involves binding to neuropilin-1 and is dynamin-dependent
(Journal article; Tidsskriftartikkel; Peer reviewed, 2023-11-09)Liver sinusoidal endothelial cells (LSEC) are scavenger cells with a remarkably high capacity for clearance of several blood-borne macromolecules and nanoparticles, including some viruses. Endocytosis in LSEC is mainly via the clathrin-coated pit mediated route, which is dynamin-dependent. LSEC can also be a site of infection and latency of betaherpesvirus, but mode of virus entry into these ... -
Mixed exposure to bacterial lipopolysaccharide and seafood proteases augments inflammatory signalling in an airway epithelial cell model (A549)
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-07-06)Seafood industry workers exhibit increased prevalence of respiratory symptoms due to exposure to bioaerosols containing a mixture of bioactive agents. In this study, a human pulmonary epithelial cell model (A549) was exposed to mixtures of bacterial lipopolysaccharide (LPS) and protease-activated receptor-2 (PAR-2) agonists H-Ser-Leu-Ile-Gly-Lys-Val-NH2 (SLIGKV-NH2), purified salmon (Salmo salar) ... -
Ocular Histopathological Findings in Semi-Domesticated Eurasian Tundra Reindeer (Rangifer tarandus tarandus) with Infectious Keratoconjunctivitis after Experimental Inoculation with Cervid Herpesvirus 2
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-09)Infectious keratoconjunctivitis (IKC) is a common transmissible ocular disease in semi-domesticated Eurasian tundra reindeer (<i>Rangifer tarandus tarandus</i>). In large outbreaks, IKC may affect tens of animals in a herd, with the most severe cases often requiring euthanasia due to the destruction of the affected eyes and permanent blindness. An experimental inoculation with cervid herpesvirus 2 ... -
Proteases from seafood as activators of protease-activated receptor-2 in airway epithelial cells
(Doctoral thesis; Doktorgradsavhandling, 2010-12-03)The work is part of a larger thematic research initiative, “Airway reactions to biological particles derived from seafood”, and was initiated in 2005 after a study conducted in the Norwegian fish industry revealed increased airway symptoms in fish industry workers. The thesis investigates the ability fish and king crab trypsins have to affect airway epithelial cells and the initiation of intracellular ... -
Purified sardine and king crab trypsin display individual differences in PAR-2-, NF-kB-, and IL-8 signaling
(Journal article; Tidsskriftartikkel; Peer reviewed, 2011-08-31)Respiratory symptoms occur in workers processing a great variety of seafood. Studies previously showed that salmon trypsin increases transcriptional activity of NF-κB and induces secretion of IL-8 from airway epithelial cells by activating PAR-2. The aim of this study was to explore if purified trypsins from king crab (Paralithodes camtschaticus) and sardine (Sardinops melanostictus) are able to ... -
The quest for a true One Health perspective of brucellosis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
The scavenger function of liver sinusoidal endothelial cells in health and disease
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-10-11)The aim of this review is to give an outline of the blood clearance function of the liver sinusoidal endothelial cells (LSECs) in health and disease. Lining the hundreds of millions of hepatic sinusoids in the human liver the LSECs are perfectly located to survey the constituents of the blood. These cells are equipped with high-affinity receptors and an intracellular vesicle transport apparatus, ...
